Acetamide Broth ISO is used in water microbiology for selective enrichment of Pseudomonas spp., especially P. aeruginosa. The medium contains acetamide as the sole carbon source, which non-fermenting gram-negative bacteria such as P. aeruginosa can deaminate, producing ammonia and increasing pH, allowing confirmation in compliance with ISO 16266.
Acetamide Broth ISO (catalogue No. 2017) is a dehydrated culture medium intended for the confirmation of Pseudomonas aeruginosa in water samples by membrane filtration. The medium uses acetamide as the sole carbon source; non-fermenting gram-negative bacteria capable of deaminating acetamide (e.g. P. aeruginosa) produce ammonia, leading to a rise in pH, which indicates presumptive presence of Pseudomonas.
Advantages
- Selective enrichment medium specific for Pseudomonas aeruginosa confirmation.
- Compliant with ISO 16266 standard for drinking and natural water testing.
- Minimal nutrient composition reduces growth of non-target flora, improving selectivity.
- Suitable for membrane filtration methodology in environmental and water quality control laboratories.
Technical specifications
| Pack size | 500 g |
| Regulation / Standard | ISO 16266 |
| Formula (g/L) | Acetamide 2.00; Magnesium sulfate 0.20; Monopotassium phosphate 1.00; Sodium chloride 0.20; Sodium molybdate 0.005; Ferrous sulfate 0.0005 (when specified) |
| Final pH (25 °C) | ≈ 7.0 ± 0.5 |
| Intended use | Selective enrichment and confirmation of Pseudomonas aeruginosa in water |
| Application | Water quality testing, environmental microbiology, microbial confirmation |
| Storage conditions | Dry place, 2–25 °C (manufacturer recommendation) |
| Chemical type | Microbial Culture Media |
| Brand | Condalab |
| Pack size | 500 g |
